Grant Gustin as Wayne Russell

Shameik Moore as Miles Morales

Hailee Steinfeld as Gwen Stacy/Spider-Woman

Kelly Marie Tran as Cindy Moon/Silk

Jake Johnson as Peter B. Parker/Spider-Man

Nicholas Cage as Peter Parker/Spider-Man Noir

John Mulaney as Peter Porker/Spider-Ham

Kimiko Glenn as Peni Parker/SP//dr

Mahershala Ali as Aaron Davis/Prowler

Kathryn Hahn as Dr. Olivia Octavius/Doc Ock

Liev Schreiber as Wilson Fisk/Kingpin

And the rest of the cast as their respective characters