Cindy Moon/Silk.

Finn Moskowitz.

Nick Fury Sr.

Henry Osborn.

Aiden Parker/White Arachnid

Amadeus Cho/Iron Spider.

Earth 2 Jason Parker/Blood Spider
Backstory:he was the survivor of the plane crash, he lived on the streets alone until he reunited with Uncle Ben, Aunt May, Jessica and Peter. During the class trip at ESU, he was bitten by a radioactive spider. He lost his uncle and adoptive cousin said he dubbed himself as the Blood Spider for his blood soaked rage through the criminal underworld.
Voice Actor:Vincent Martella.

Miguel O'Hara/Spider-Man 2099

Earth 14 Jason Parker/Scarlet Spider Noir
Backstory:he was a young man with a bright future until the vulture killed his uncle Ben which prompted him to be the Scarlet Spider Noir along with Peter becoming Spider-Man Noir.
Voice Actor:Christopher Daniel Barnes.

Tory Thompson

Stiles Octavius/Superior Spider-Man

Oliver Octavius/Superior Octopus

Earth 16 Jason Parker/Spider-Man II
Backstory:After Peter's death at the water tower with Norman, Jason chose to abandon the Scarlet Spider identity to embrace the destiny as Spider-Man and he left Midtown High to go to Horizon High (one of prestigious schools for the gifted minds) and was mentored by Oliver Octavius (the brother of Doc Ock), he was given a internship at Stark Industries and became a member of the Avengers thus taking on an apprentice in Miles Morales.
Voice Actor:Nolan North.

Clint Barton/Hawkeye.

Thor Odinson

Lieutenant Shane Walsh.

Charlie Barton/Green Arrow

Dean Parker/Red Hood