It's been a month since the singularity and everything has changed since then. Cisco is working with the CCPD to work on a way to stop metahumans without needing to depend on the Flash, Caitlin has gotten a job at Mercury Labs recently as Barry has isolated himself and Stein has been with his wife since.

Right now, Peter is showing Caitlin around the rest Mercury labs. "On this floor is where we have protein structure, rDNA, chromatography, transgenic testing, that's dr. Conners labs with X-ray video." Peter explains to her when she notices something.

"What's that?" Caitlin asks and sees a device kept behind glass. It just looks like a enhanced version of some sporting t-shirt cannon with electronic applications.

"Oh, that's something me and Conners worked on my first year here. We call it the Ganali device, it's basically a simple idea. We first load it with any antigen, then it will be shot up into the atmosphere turning into a cloud that could cover from a neighborhood to all across the city. Theoretically, it could even cure polio in an afternoon." Peter explains to Caitlin and it surprises her.

"That's incredible." Caitlin states to him and Peter disagrees.

"Well, It was. But then when it was finished, we were putting together pros and cons, and then we realized that it could be loaded with a toxin, or if you wanted to back out. You can't exactly run from a cloud, so we just keep it here. Just in case something were to come up one day." Peter explains when an alert goes off in his phone.

"Action time?" Caitlin asks him. "Looks like there's an attempting breakout happening at Iron Heights." Peter says and she runs off to her lab to get everything set up.

-

Peter swings towards the prison as Caitlin is in comms. "Talk to me, Cait." Peter tells her as he lands on a police car to get him there faster.

"Okay, It seems like someone planned a coordinated attack. All of the cell blocks have been breached, but the police have them handled, but the cell with your enemies..." Caitlin tells him and he sees this is bad.

"Okay, I'll lock down the psycho's in costumes, hopefully I'll get reception down there." Peter tells her and makes his way down to where he knows they're holding his enemies.

Peter enters the sewers and looks around. "Hey, Everything going okay?" Caitlin asks him as he's not getting anything so far.

"Yeah, everything okay so far." He tells her when his senses go off. "Oh no."

He quickly moves to dodge a mechanical tentacle and looks to see Otto. "Look who we have here, it's Octopus." Peter says as he exits the shadows to reveal his new mechanical arms he must've been working on ever since he escaped with the Trickster.

"Do not call me that!" Otto tells Peter.

"Hey, if the tentical footwear fits." Peter quips and gets ready for action. "So why did you return here if you already escaped?" Peter asks, not understanding why he's back here.

"I have a few associates to reunite with." Otto explains when more figure come from the corridors.

Martin Li, mr. Negative. Name explains most of it, he uses his dark energy power to fight.

Mac Gargan, Scorpion. An entire suit of armor with a tail with poison inside.

Adrian Toomes, Vulture. A small arms weapons dealer who was Peter's fist enemy when he started out.

Max Dillon, Electro. Peter's first powered enemy before metahumans started appearing everywhere.

Kraven the Hunter. Basically a psycho hunter who thought Peter's head would look good on a pointy stick.

"Well look at this, you let all of my past enemies out of their cells." Peter jokes and wonders how he plans on fighting all six of his greatest enemies at once.

"I give you the Sinister Six!" Otto introduces his team to Peter.

"Wow, that's a terrible name. All that build up for so little." Peter states when Rhino charges at him and he easily dodged it.

"Get him! All of you!" Otto tells his team and they all run at him one at a time. Kraven tries hitting him with his sphere and Peter dodges it like nothing and lands on the roof. What he didn't expect was Vulture flying at him and grabs him. "Toomes?!" Peter asks hating birds now.

"Long time no see, pest!" Vulture states and Peter just webs his mask and drops him. He then dodges a blast from Electro and Rhino charging at him.

Mr. Negative sends a blast at him and sends him into the wall. "Okay, that was a good one." Peter compliments when Electro blasts at him again sending him across the room.

Kraven grabs him and throws him into Otto's clutches. "You guys give up yet?" Peter asks them knowing this isn't a fight he's going to win in this environment.

"I applaud your bravado even as I question your intelligence. Coming here without a team, Flash dump you after the singularity?" Otto asks since Flash has only been seen once in a while and is likely dealing with the breakout upstairs.

"Pe-er? I -re y-u.." the signal starts breaking and Peter can't hear her.

"And I'm blocking all communications from getting out. Rhino! Remove his mask and show me who this nuisance is." Otto tells the living tank and before he had the chance, Peter leaps back up and runs back into the tunnel he came from.

"Cait, can you hear me? I'm gonna need you to call Arrow, maybe Flash. I'll even take Vixen if you can get her." Peter states not being picky on who to contact as the six super villains keep following.

"Looks like Ock was right. I guess I'm on my own. Caitlin is going to kill me." Peter states as the enemies are trying to track him and gets an idea.

The team chase Spider-Man to a corridor that separates into different places. "Which way, fearless leader?" Negative asks mockingly.

"Sinister Six, come out and play!" Peter calls them out.

"Kraven, you have superior tracking skills, don't you? Where is he?" Otto asks Kraven.

"I can't tell. It's like an echo chamber in here, and his scent seems to be everywhere." Kraven responds to him.

"Hey! Is he saying I stink? Yet again, I never did wash this suit after my run in with Grodd." Peter realizes and gets back on topic to mock these guys.

"Here's a question for you. How many members of the Sinister Six does it take to change a lightbulb? The answer is six. Four of them to hold down Electro, and one of them to screw the lightbulb into his mouth!" Peter mocks them and Electro laughs at that remark.

"You guys wanna see that? It actually does work." Electro asks his team members and gets angry stares from all of them. "Sorry." He tells them, apologetically.

"Enough! There are six tunnels and six of us. If we each take one, he cannot possibly escape." Otto tells them and they each take a tunnel to find him.

Rhino is searching when he suddenly gets webbed into the wall.

"It'll take even you a minute to get out of that webbing, big guy." Peter informs him when a mechanical tentacle gets him.

"I'm seriously hating my luck today." Peter states as the other members show up and Kraven frees Rhino.

"Someone captures my quarry." Kraven states to the other members. "But the fun isn't over yet. I can still spark things up, and him." Electro responds to them. "Let me fill his entire body with venom." Scorpion states, ready to finish him off.

"No! Let me absorb all of his energy, killing him." Negative states, seriously wanting to feel Peter's strength and will power.

"No. Let me squish him." Rhino informs them when Otto stops all of them.

"The only prize here will be his unmasking." Otto states and Vulture agrees since they both have been waiting over fifteen years to learn his identity.

But Peter acts quickly and leaps at Rhino to take down their biggest weapon. "Stop him!" Otto tells Electro and he aims at Spider-Man, but accidentally hits Rhino as Peter got out of the way.

"Watch who you're zapping!" Rhino states and runs into Electro, sending him flying into Vulture.

"Stop fighting each other and stop him!" Otto commands the team.

"This is like the Six Stooges instead of the three." Peter jokes at them. "I mean, I thought you all broke out of this prison to destroy me. It seems like you're all trying to destroy each other to kill me. Did none of you realize there's only one Spider-Man and six members?" Peter wonders.

"I mean, I know some of you are too stupid to remember that, right horn head?" Peter asks Rhino.

"Are you talking to me?!" Rhino asks probing Peter's point.

"Well, is there anyone else in your team zoo with a horn on them?" Peter asks him.

Rhino gets seriously angry and charges at Peter again and runs into the pipes in the sewers. The pipes then burst and the water hits all of the members, including Electro shocking everyone.

"Water and electricity, they never mix." Peter informs to them all and makes his escape.

Peter makes his getaway when suddenly Rhino comes out of the ground and the rest of the sinister suddenly follow afterward.

"Did you really think you'd get away that easily?" Otto asks as the citizens start running for it.

"To be completely honest, yes." Peter states honestly to him and starts fighting off all the members yet again. But luckily for Peter, they're not well with working together. Rhino then comes charging at him from behind and Peter's senses warn him ahead.

"I'm gonna squash you like a bug!" Rhino promises Peter and he just dodges him as he runs into Otto instead.

"Come on, man, how many times have we had this conversation? I'm an arachnid. Not a bug!" Peter informs Rhino for the dozenth time and webs his head into the ground.

Vulture comes flying up behind him and shoots his weapons at him. "Hey, since when did you have those?" Peter questions since Adrian is usually just using his wings, never weaponry.

"You know, for a Vulture you sure fly like a chicken." Peter jokes as the weapons just hit Electro and Rhino.

Mr. Negative goes in to fight, but Peter quickly equips a web grenade to his back, stopping him and as it explodes, it also traps Kraven.

"That should be all of them." Peter states as he looks over the villains and sees two of them missing.

"Hey, Spider-Twerp! I'm still here!" Electro informs Peter and shoots a blast of lightning in his direction.

"You see?! Now that's one I haven't heard before, and was clever." Peter states while dodging all of the lightning blasts and Scorpion's sting attacks.

"This time, you loose, Spider-Man." scorpion tells him when a flash of lightning comes in and knocks the Scorpion to the ground. They look to see the Flash.

"Hey fellas. I don't think we've been properly introduced. Names Flash." Barry introduces himself and speeds to help Peter back up.

"You okay, big man?" Barry asks his friend he hasn't seen in a while.

"You know, a broken this. Might've bruised that. Normal day in the office. Now it's just the matter of defeating the six of my deadliest enemies at the same time." Peter informs Barry.

"Well, I already took out the Stinger." Barry states since Scorpion is out cold.

"Okay, that's one, then there were five." Peter responds as they all stand back up.

Otto sends a tentacle at them, but Peter quickly stops it and uses it to run up and kick Otto in the face. Barry speeds off and starts fighting mr. Negative.

"So, if you're mr. Negative, is there a Mrs. Positive?" Barry wonders as Peter starts fighting off Kraven.

"Come on, man. I used that one years ago." Peter states to him and webs Kraven to the wall. Rhino comes up and slams Peter into the wall.

"Your failure is sublime." Otto states to him.

"Even with the Flash, you're nothing against my Sinister Six!" He informs them both.

"Wait, Sinister Six? That's what you're calling them?" Barry questions him. "I mean, I could've come up with something better." Barry states and speed up on him and Peter does the same thing from behind. They combine their strengths and knock Otto out and Peter rips his mechanical limbs off of their placements.

"Not so sinister anymore." Peter states as all of their members are down and accounted for. But once he turned around, and sees Barry has vanished.

-

Back at Mercury labs, Caitlin is helping Curt Conners with his research to grow back people's limbs that have been lost by using Lizard DNA.

"What you see here, dr. Snow, is a computer model of a lizard. Now, many of these wonderful creatures have so brilliantly adapted that they can regenerate entire limbs at will." Conners informs her and she understands why he would be interested in doing so since he lost his right arm.

"Me and dr. Parker are trying to harness the capability and transfer it into our host subject, Freddy the three-legged mouse." Conners explains to her and uploads the algorithm that she put together to help them. "System ready for gene insertion." The computer informs them.

"It's preempting the proteins." Conners tells her and they wait to see if it's working and after a few trials and errors, the peptide algorithm was accepted and the computer Freddy regrew his missing limb.

"It worked." Caitlin states and chuckle.

Conners shows to her the two mouse subjects as Peter was able to join them after cleaning himself up from the fight. "Meet Freddy and Wilma, our three legged mice." Peter introduces Caitlin to their mice.

Conners picks up Freddy and Peter takes him out of his hand and Conners has the serum ready for injection. "Let's be careful. We don't want to begin human trials just yet." Conners jokes as they inject Freddy and place him back in his cage and they just have to wait and see if it worked.